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reakcia polymerázová reťazová

Subject h.	reakcia polymerázová reťazová
Subject h.	Polymerase Chain Reaction
Entry terms	PCR PCR ukotvená PCR zakotvená PCR inverzná PCR obrátená PCR nested PCR hniezdová
English X references	Anchored PCR Inverse PCR Nested PCR PCR
Scope note in English	In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
See also reference (FX) in Slovak	reakcia ligázová reťazová miesta sekvenčné označené
See also reference (FX) in English	Ligase Chain Reaction Sequence Tagged Sites
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