0012979 2022 RIV GB eng J
Šašinková, Markéta - Heřman, P. - Holoubek, Aleš - Strachotová, D. - Otevřelová, Petra - Grebeňová, Dana - Kuželová, Kateřina - Brodská, Barbora^G
NSC348884 cytotoxicity is not mediated by inhibition of nucleophosmin oligomerization.
Scientific reports. Vol. 11, no. 1 (2021), art. no. 1084. ISSN 2045-2322
Grant: Grantová agentura ČR GA19-04099S
Institucionální podpora: RVO:00023736
Klíčová slova: nucleophosmin * oligomerization * mutation * NSC348884 * adhesion
Cite Score: 6.900, rok: 2021; SJR: 1.005, rok: 2021; IF: 4.996, rok: 2021
DOI: 10.1101/2020.02.06.936666

Anotace: Oligomerization of the nucleolar phosphoprotein nucleophosmin (NPM) is mediated by its N-terminal 50 domain. We introduce a reliable set of complementary methods for monitoring NPM 55 oligomerization in both cell lysates and live cells. Using this methodological background we show that 56 a putative inhibitor of NPM oligomerization, NSC348884, does not prevent formation of NPM 57 oligomers in leukemia cells. Instead, we reveal that the observed cytotoxic effect of NSC348884 is 58 associated with changes in cell adhesion signaling.