0013457 2023 RIV CZ eng J
Smetana, Karel^G - Mikulenková, Dana - Karban, J. - Trněný, M.
RNA concentration and content in the nucleoli and cytoplasmic rim in differentiating lymphocytes of patients suffering from B chronic lymphocytic leukaemia: a cytochemical note.
Folia biologica. Roč. 68, č. 1 (2022), s. 40-44. ISSN 0015-5500
Institucionální podpora: RVO:00023736
Klíčová slova: cytoplasmic rim * B chronic lymphocytic leukaemia * nucleoli
Cite Score: 1.800, rok: 2022; SJR: 0.294, rok: 2022; IF: 0.6, rok: 2022

Anotace: Nucleolar RNA optical density (concentration) measurements at the single cell level indicated that differentiation of lymphocytes is accompanied by a slightly decreased nucleolar RNA concentration in contrast to the cytoplasmic rim around the nucleus. On the other hand, the nucleolar size was markedly reduced and the cytoplasmic rim surrounding the nucleus was reduced only weakly. Concerning the calculated rough estimate of the RNA content, the differentiation induced its larger decrease in the nucleoli than in the cytoplasmic rim. These observations indicated that the nucleolar RNA concentration and RNA content together with the nucleolar morphology are more sensitive markers of the differentiation process than the RNA concentration and content in the cytoplasm.