DNA methylation validation methods: a coherent review with practical comparison

0012760 2020 RIV GB eng J
Šestáková, Šárka - Šálek, Cyril - Remešová, Hana^G
DNA methylation validation methods: a coherent review with practical comparison.
Biological procedures online. Vol. 21, no. 1 (2019), art. no. 19. ISSN 1480-9222
Grant: Ministerstvo zdravotnictví IP
Klíčová slova: DNA methylation * validation methods * MSRE * pyrosequencing * MS-HRM * qMSP
Cite Score: 6.400, rok: 2019; SJR: 1.038, rok: 2019; IF: 2.711, rok: 2019
DOI: 10.1186/s12575-019-0107-z

Anotace: Here, we present a practical overview of four commonly used validation methods for DNA methylation assessment: methylation specific restriction endonucleases (MSRE) analysis, pyrosequencing, methylation specific high-resolution DNA melting (MS-HRM) and quantitative methylation specific polymerase chain reaction (qMSP). Using these methods, we measured DNA methylation levels of three loci in human genome among which one was highly methylated, one intermediately methylated and one unmethylated. We compared the methods in terms of primer design demands, methods’ feasibility, accuracy, time and money consumption, and usability for clinical diagnostics.