0013710 RIV IT eng J
Smetana, Karel^G - Mikulenková, Dana - Karban, J. - Trněný, M.
To the ring-shaped nucleolus seen by microscopy using human lymphocytes of blood donors and chronic lymphocytic leukemia patients.
European journal of histochemistry. Vol. 68, no. 3 (2024), art. no. 4075. ISSN 1121-760X
Institucionální podpora: RVO:00023736
Klíčová slova: human lymphocytes * ring-shaped nucleolus * B chronic lymphocytic leukemia
Cite Score: 4.100, rok: 2024; SJR: 0.541, rok: 2024; IF: 2.1, rok: 2024
DOI: 10.4081/ejh.2024.4075

Anotace: The present study was undertaken to provide more information on the peripheral RNA containing ring of ring-shaped nucleoli (RSNo). Human lymphocytes of blood donors and patients suffering from B chronic lymphocytic leukemia mostly characterized by RSNo represented very convenient cell models for such study. According to the light microscopy the peripheral RNA ring possessed several highly condensed foci. Such regions represented accumulated dense RNA fibrillar components (DFCs) seen by the electron microscopy. In contrary, the incidence of dense granular RNA-containing components (GCs) in surrounding portions of the RNA ring was small. Thus, the structural and morphological organization of the peripheral RNA ring of RSNo apparently reflects sites of micro-segregated foci of DFCs and a small incidence of GCs. That structural organization of the peripheral RNA ring of RSNo appeared to be a prerequisite for further regressive nucleolar changes resulting in the development of micronucleoli in terminal lymphocytes.