Number of the records: 1
reakcia polymerázová reťazová
Record number d016133 Date 06.06.2025 Type M - MESH Topical term reakcia polymerázová reťazová Other term English (Pseudonym) Anchored PCR
English (Pseudonym) Inverse PCR
English (Pseudonym) Nested PCR
English (Pseudonym) PCR
Slovak (Pseudonym) PCR
Slovak (Pseudonym) PCR ukotvená
Slovak (Pseudonym) PCR zakotvená
Slovak (Pseudonym) PCR inverzná
Slovak (Pseudonym) PCR obrátená
Slovak (Pseudonym) PCR nested
Slovak (Pseudonym) PCR hniezdová
See also (Skutočné meno) miesta sekvenčné označené
(Skutočné meno) reakcia ligázová reťazová
(Later heading) reakcia ligázová reťazová
UDC E05.393.620.500 Note In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships. 
subject heading
Number of the records: 1