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reakcia ligázová reťazová

Record number	d022521
Date	06.06.2025
Type	M
Topical term	reakcia ligázová reťazová
Other term	Slovak (Pseudonym) LCR
See also	(Later heading) reakcia polymerázová reťazová (Skutočné meno) reakcia polymerázová reťazová
UDC	E05.393.620.311
Note	A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.

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