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imunoprecipitácia chromatínu

Record number	d047369
Date	06.06.2025
Type	M - MESH
Topical term	imunoprecipitácia chromatínu
Other term	English (Pseudonym) Immunoprecipitation, Chromatin
UDC	E05.393.170E05.478.605.160
Note	A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of CHROMATIN to crosslink the DNA-BINDING PROTEINS to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific ANTIBODIES. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing.

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Number of the records: 1