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purifikácia tandemová afinitná
Subject h. purifikácia tandemová afinitná Subject h. Tandem Affinity Purification Entry terms chromatografia tandemová afinitná Scope note in English A technique used to isolate specific interacting partners of MACROMOLECULAR COMPLEXES that have been assembled with a tagged component (bait) that interacts with other partners in the complex. The interacting bait component is labeled with a dual-affinity tag that allows for capture of the complex by IMMUNOPRECIPITATION, followed by purification of only the partners of the complex interacting with the bait by affinity chromatography. subject heading
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