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ELISA

  1. Subject h.ELISA
    Subject h.Enzyme-Linked Immunosorbent Assay
    Entry termsanalýza imuno-enzýmová
    analýzy enzýmové imunoadsorbentové
    enzýmom značená imunosorbčná metóda
    English X referencesELISA
    Scope note in EnglishAn immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
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