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reakcia polymerázová reťazová

  1. Subject h.reakcia polymerázová reťazová
    Subject h.Polymerase Chain Reaction
    Entry termsPCR
    PCR ukotvená
    PCR zakotvená
    PCR inverzná
    PCR obrátená
    PCR nested
    PCR hniezdová
    English X referencesAnchored PCR
    Inverse PCR
    Nested PCR
    PCR
    Scope note in EnglishIn vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
    See also reference (FX) in Slovak reakcia ligázová reťazová
    miesta sekvenčné označené
    See also reference (FX) in English Ligase Chain Reaction
    Sequence Tagged Sites
    Links (452) - ARTICLES
    (2) - MeSH descriptor
    (53) - CiBaMed
    (7) - ELECTRONIC RESOURCES
    (60) - BOOKS
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