reakcia polymerázová reťazová

Polymerase Chain Reaction

PCR
PCR ukotvená
PCR zakotvená
PCR inverzná
PCR obrátená
PCR nested
PCR hniezdová

Anchored PCR
Inverse PCR
Nested PCR
PCR

In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.

reakcia ligázová reťazová
miesta sekvenčné označené

Ligase Chain Reaction
Sequence Tagged Sites